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In this study, we evaluated the genetic relationships of fourty seven Pestalotiopsis these isolates collected from diseased sweet persimon in various places in southern part of Korea using RAPD (Randomly Amplified Polymorphic DNAs) method. As a result of the amplification, eight primers showed total of 86 bands ranging from 0.3 Kb to 3.2 Kb. Among those 86 bands, 84 polymorphic bands were used for bionominal matrix code (0, 1), and UPGMA dendrogram analysis. Similarities among the compared isolates ranged from below 60% to more than 95%. Most of the compared isolates showed 50∼80% similarities. The number of isolate pairs which showed more than 80% similarity were 248. The number of isolate pairs which showed 50∼80% similarity were 789, and the number of isolate pairs which showed below 50% similarity were 21. Isolate SP-21 (No. 9) showed below 50% similarity with all the isolates compared. At 50% similarity level, all the isolates compared, except isolate SP-21 (No. 9), were included in one big group. At 65% similarity level, all the isolates compared, except isolate SP-21 (No. 9), were divided into three different groups. At 75% similarity level, all the isolates compared, except isolates SP-47 (No. 23) and SP-21 (No. 9), were divided into six different groups.
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